FP7 logo euThe ESNATS project has received funding from the European Community's Seventh Framework Programme (FP7/2007-2013) under grant agreement n° HEALTH-F5-2008-201619

Embryonic Stem cell-based Novel Alternative Testing Strategies

Partners: Université de Genève (UNIGE)

Université de Genève The Pathology Department of the University of Geneva conducts research into anatomical and clinical correlations in human pathology, and in the fields of cell biology (with a particular focus on stem cells), general immunology and immunopathology. The major contribution of UNIGE to the project consists i)in the genetic engineering of embryonic stem cells to generate reporter cell lines; ii) in the analysis of the effect of neurotoxic compounds on minibrains, and iii) in the development of innovative neurotoxicity assays.

Key staff involved in the project

Karl-Heinz Krause, Prof.
Karl-Heinz KrauseProfessor Krause's research areas: Biology of ageing, NOX family of ROS-generating NADPH oxidases, neuronal differentiation of embryonic stem cells. Reactive oxygen species (ROS) play a key role in aging and the development of age-associated diseases. Members of the NOX family of superoxide generating NADPH oxidases. Members of this enzyme family are found in virtually all tissues of our organism, where they contribute to redox-dependent physiological processes, but also pathophysiologically relevant oxidative stress. Embryonic stem cells are multipotent stem cells with a major potential for basic science and for clinical applications. We are particularly interested in the neuronal differentiation of embryonic stem cells, aiming to understand basic mechanisms of differentiation and to advance towards cell therapy of central nervous system diseases.

Olivier Preynat-Seauve , PhD.
Olivier Preynat-SeauveOlivier Preynat-Seauve's research area: embryonic stem cells, neural differentiation of embryonic stem cells, immunology. Our research is focused on the neural differentiation of embryonic stem cells. We have developed a method based on air/liquid interface allowing differentiation in three dimensions, generating a tissue sharing characteristics with human fetal brain. We also work on a flow cytometry - based approach to characterize the phylogeny of all the cell subpopulations generated from stem cells during neural differentiation. Finally, we are interested in the immunogenicity of stem cell-derived progenitor cells, focusing on the allorecognition by T lymphocytes, NK cells and the role of immunosuppressive drugs.

Luc Stoppini, Dr.
Luc StoppiniSenior scientist, originally developed organotypic culture systems of CNS tissue on an air-liquid interface. He is an expert in the development of 3D tissue culture systems and their application to functional recording using microelectrode arrays. He brings this expertise to the group and will be responsible for this aspect of the project.

Further information:

Karl-Heinz.Krause at medecine.unige.ch

Preynat-Seauve at medecine.unige.ch

Faculty of medicine
Department of Pathology and Immunology
1, rue Michel Servet
CH-1211 Geneva Switzerland

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